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Kultivace Vitis vinifera v podmínkách in vitro a převod do in vivo podmínek
Bechyňová, Soňa
I examined in vitro behaviour three table varieties of grapes and Vitis rootstock Craciunel 2 during different micropropagation stages and different rhizogenezis media composition in vitro conditions. The experiment held the place in Lednice na Morave in 2018. So far have been achieved best results of 90% rooting success with DKW medium with the addition of growth regulators at this concentration of 0,2 mg.l-1 NAA + 0,2 mg.l-1 IAA + 1 mg.l-1 IBA. Multiplication protocol suitable for use in horticultural practice were optimized for Craciunel 2. Different media factors affect rooting have been determined.
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Micropropagation of Satureja Montana L.
Zunová, Tereza ; Fernández Cusimamani, Eloy (advisor) ; Michaela, Michaela (referee)
Winter savory (Satureja montana L.) is a plant occurring in the Mediterranean and the Black Sea region. For its high content of essential oils is widely used in culinary and traditional medicine.
Savory is propagated generative and vegetative. Seeds germinate slowly, and therefore, the vegetative propagation is faster, especially in in vitro conditions may be propagated a large amount of plant material in a short time.
The aim of this thesis was to investigate the growth effect of cytokinins on regeneration and growth plants from nodal segments of S. montana L.
Nodal segments were cultured on six variants of medium supplemented with 0.5; 1 and 1.5 mg/l 6-benzylaminopurine (BAP) and 0.5; 1 and 1.5 mg/l kinetin (KIN). Variant without growth regulators served as a control. Culture conditions were 25/20 °C day/night, light intensity 3000 lx and daily period of 16/8 hours. After 6 weeks of measurements were evaluated parameters such as: number of shoots, plant height, the number of newly created nodes and the number and length of roots.
In respect of the coefficient of micropropagation (i.e. the number of newly created nodes on the plant) was statistically significantly best variant medium supplemented with 1.5 mg/l KIN where on average create 8.88 +/- 2.87 nodes. At the height of the plant which was highest in the variant with a concentration 1.5 mg/l KIN (2.27 +/- 1.33) and the number of shoots with highest average value in a concentration 1 mg/l BAP (2.87 +/- 1.19) encore of growth regulators were not statistically proven. High concentrations of BAP and KIN inhibited the induction of roots and from variants transferred to ex vitro conditions were regenerated 46 % plants.
Adding BAP and KIN into the medium has an influence on plant regeneration of Satureja montana L.
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Micropropagation of Lippia dulcis Trev.
Zubíková, Alžběta ; Fernández Cusimamani, Eloy (advisor) ; Michaela, Michaela (referee)
Lippia dulcis Trev. (Verbenacea) is a perennial herb with a creeping stem from Central America. It is known for its healing properties for problems with the respiratory or digestive system, but also its sweet taste, which causes terpene hernandulcin because of which the leaves of Lippia dulcis Trev. are 1,000 times sweeter than sugar. Generatively, the plant reproduces by seeds and vegetatively by cuttings.
The main objective of this study was to investigate the influence of cytokinins kinetin (KIN) and 6-benzylaminopurine (BAP) with the addition of auxin indole-3-acetic acid (IAA) on the growth and regeneration of plants from nodal segments Lippia dulcis Trev. in vitro conditions.
Nodal segments were cultured on 6-variants of MS medium supplemented with growth regulators at concentrations of 0.5; 0.75 and 1.0 mg / l BAP and KIN with combination 0.5 mg / l IAA and one variant of pure MS medium as a control. Nodal explants were cultured for 35 days (the time of subculturing) and measured every 7 days. The following parameters were evaluated: regeneration, number of shoots, length of shoots, number of nodes, number and length of roots.
The measurement results show that a combination of growth regulators auxin-cytokinin have no positive influence on the growth and regeneration Lippia dulcis Trev. in vitro conditions. The best regeneration (100%), growth (on an average length of the shoots 3.05 +- 1.42) and the highest number of newly created points (5.79 +- 2.4) occurred at the explants cultured on a clean MS medium without addition of growth regulators. Among the variant with pure MS and media variants with the addition of growth regulators are statistically significat defferences, but without a favorable influence on the development of nodal explants.
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